Pleosporales - CBS - KNAW

Fungal Diversity
times furcate pedicel, up to 13 μm long, ocular chamber
not observed (Fig. 47f, g, h and k). Ascospores 16–20×9–
11 μm (x ¼ 18 10mm, n=10), biseriate, ellipsoidal, pale
orange-brown, 1-distoseptate, with prominent swelling at
the septum, containing refractive globules, smooth (Fig. 47i,
jandl).
Anamorph: The following description is from Calatayud et
al. (2001).
Conidiomata pycnidial, arising in galls together with the
ascomata, immersed, ca. 100–200 μm diam.; wall dark
brown throughout, composed of 2–5 layers of angular to
laterally compressed cells; cells relatively large, ca. 8–
16 μm diam. in superficial view. Conidiophores formed by
1–3 cells, frequently branched and with the uppermost cells
bearing 1–4 conidiogenous cells; cells±cylindrical, hyaline
except at the base, which are sometimes pale brown, 7–15×
3–4 μm. Conidiogenous cells tapered towards the apex, 14–
18×3–4 μm. Conidia 5–7×1.5–2 μm. Vegetative hyphae
hyaline.
Material examined: SPAIN, Andalucía, Province, Jaén,
Andújar, lichenicolous on Leptochidium albociliatum
(Desm.) M. Choisy on acid volcanic rock, 19 Apr. 2000,
V. Calatayud (MA-Lichen 12715, holotype).
Notes
Morphology
Lichenopyrenis was formally established by Calatayud et
al. (2001) based on its “perithecioid ascomata with peridium
comprising compressed cells, fissitunicate and J- asci, wide
hamathecium filaments, and 1-septate pale orange-brown
ascospores with distoseptate thickenings at maturity”, andis
monotypic with L. galligena. The genus was temporarily
assigned to Pleomassariaceae. Lichenopyrenis galligena is a
parasite of lichens, occurring in galls in the thallus of the host
(Calatayud et al. 2001).
Phylogenetic study
None.
Concluding remarks
This is one of the few species that are parasitic on
lichens. The most comparable species are Parapyrenis
lichenicola Aptroot & Diederich and Lacrymospora parasitica
Aptroot (both in Requienellaceae, Pyrenulales) as
well as some species from Dacampiaceae. The peridium
structure, cellular pseudoparaphyses, distoseptate and
smooth, orange-brown ascospores as well as the anamorphic
stage of Lichenopyrenis can easily distinguish from all
of them (Calatayud et al. 2001).
Lineolata Kohlm. & Volkm.-Kohlm., Mycol. Res. 94: 687
(1990). (Pleosporales, genera incertae sedis)
Generic description
Habitat marine, saprobic (or perthophytic?). Ascomata
medium-sized, gregarious, immersed to erumpent, obpyriform,
ostiolate, papillate. Peridium thin, comprising two
types of cells; outer cells thick stratum pseudostromatic,
inner stratum thin, composed of a few layers of hyaline
cells of textura angularis. Hamathecium of dense, long
trabeculate pseudoparaphyses, embedded in mucilage,
anastomosing and septate. Asci 8-spored, bitunicate, cylindrical,
with short pedicels, with an ocular chamber.
Ascospores uniseriate to partially overlapping, ellipsoidal,
dark brown, 1-septate.
Anamorphs reported for genus: none.
Literature: Kohlmeyer and Kohlmeyer 1966; Kohlmeyer
and Volkmann-Kohlmeyer 1990.
Type species
Lineolata rhizophorae (Kohlm. & E. Kohlm.) Kohlm. &
Volkm.-Kohlm., Mycol. Res. 94: 688 (1990). (Fig. 48)
≡ Didymosphaeria rhizophorae Kohlm. & E. Kohlm.
Icones Fungorum Maris (Lehre) 4 & 5: tab. 62a (1967).
Ascomata 300–490 μm high×200–360 μm diam.,
gregarious, immersed to erumpent, obpyriform, ostiolate,
papillate, subcarbonaceous to subcoriaceous, blackish
brown (Fig. 48a). Peridium 37–45 μm thick, comprising
two types of cells; outer cells thick stratum pseudostromatic,
composed of irregular or roundish, dark brown cells,
on the outside with a more or less recognizable hyphal
structure, enclosing some decaying cells of the host, inner
stratum thin, composed of four or five layers of hyaline,
polygonal, elongate, thin-walled cells with large lumina,
merging into the pseudoparaphyses. Hamathecium of
dense, long trabeculate pseudoparaphyses, 1–1.5 μm broad,
embedded in mucilage, anastomosing and septate. Asci
150–175×14–17.5 μm, 8-spored, bitunicate, cylindrical,
with short pedicels, with an ocular chamber (Fig. 48b).
Ascospores 23–32(−33)×9–12 μm, uniseriate to partially
overlapping, ellipsoid, dark brown, 1-septate, not or slightly
constricted at the septum, striate by delicate costae that run
parallel or in a slight angle to the longitudinal axis of the
ascospore (Fig. 48c, d, e and f) (adapted from Kohlmeyer
and Kohlmeyer 1979).
Anamorph: none reported.
Material examined: US, Florida, Middle Torch Key, on
Rhizophora mangle, 21 Nov. 1965, J. Kohlmeyer (Herb. J.
Kohlmeyer No. 2390b, isotype); Pirate Grove Key, on R.
mangle, 5 Jan. 1964 (Herb. J. Kohlmeyer No. 1721 paratype);
Florida, Virginia Key, on R. mangle, 1 Jan. 1964, leg.
E. Kohlmeyer (Herb. J. Kohlmeyer No. 1751 paratype);
Florida, Torch Key, on R. mangle, 20 Nov. 1965, leg. J.
Kohlmeyer (Herb. J. Kohlmeyer No. 2423 paratype).