Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Step 5 – Programming the Plate Document MethodProgrammingMethods forAbsolute orRelativeQuantificationNote: The following procedure describes how to configure only the basic features ofthe method: thermal cycler conditions, sample volume, and data collection options.To further customize the method for the plate document, click in the Instrumenttab and refer to the online help for instructions on configuring the auto increment andramp rate values.1. In the SDS software, select the Instrument tab of the plate document.2. If necessary, select or de-select the 9600 Emulation check box.Note: When the 9600 Emulation check box is checked, the SDS softwarereduces the ramp rate of the 7900HT instrument to match that of theABI PRISM ® 7700 Sequence Detection System instrument.3. Modify the default thermal profile for the method as needed:To…adjust stepparameters(time/temp)Then…select a field value, enter a new value, and click anywhereoutside of the field.Temperature field(4 to 99.9 °C)Time field(0:01 to 98:59 min)add a hold, cycleset, or step1. Click the step to the left of the location you want to place thenew stage.2. Click or .The software inserts the stage into the thermal profile.Note: To add a step to a stage, select the step to the left of thelocation you want to place the step and click .Selected stepNew stepappears hereremove a step1. Click the step you want to remove.The software highlights the selected step.2. Click to remove the step from the profile.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 3-21DRAFTSeptember 1, 2004 11:39 am, CH_Run.fm