Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 7Maintaining the Instrument5. Click .The software creates a plate document with the attributes for a background run.IMPORTANT! Do not modify the background plate document. The method for aBackground run is coded into the SDS software and consists of a single hold at60 °C for 2 min. Because the plate contains only deionized water, the platedocument does not require sample or detector labels.6. Save the plate document:a. Click (or select File > Save).b. In the File name field of the Save dialog box, enter:Background_For example, the file name for a background plate run on May 31, 2001would be: Background_053101.c. Click .The software saves the plate document and is now configured for theBackground run.7. Continue with “Running the Prepared Background Plate or TaqMan LowDensity Array” below.Running the Prepared Background Plate or TaqMan Low Density ArrayPerforming theBackground Run1. Load the background plate into the 7900HT instrument:a. In the plate document in the SDS software, select the Instrument tab.b. In the lower portion tab, select the Real-Time tab.c. In the Real-Time tab of the Instrument tab, click .d. Place the background plate or Low Density Array into the instrument trayas shown below.Well A1Position the plate so that the bar codefaces towards the front of the instrumentWell A1Position the Low Density Array so that the barcode faces towards the front of the instrumentNote: The A1 position is in the top-left side of the instrument tray.7-18 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm