Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

adjusting step parameters 3-21configuring data collection options 3-22programming 3-20 to 3-23removing a step 3-21setting the sample volume 3-22minimumgene expression level 6-33modes of operation 1-17automated 1-18database 1-18stand-alone 1-17mRNA targets, number per well 4-10Nno template control (NTC)detector task 3-16NTC calls applying 5-15NTC calls verifying 5-16Number of targets per well 4-10O↓ 6-33openinginstrument tray (Automation ControllerSoftware) 4-44instrument tray (SDS software) 4-29plate documents 2-19operating systemsupported 1-6upgrading 7-55operating the 7900HT instrumentpower switch 2-4optimizing primer and probe concentrations B-3outliersallelic discrimination 5-8automatic outlier removal 6-19outlying amplificationmulti-reporter experiments 6-19Ppaneshiding 2-21maximizing/minimizing 2-22resizing 2-21, 2-22showing 2-21passive referencesetting 3-18PCR5´ Nuclease Assay D-2Geometric (Exponential) Phase D-4kinetic analysis of D-4Linear Phase D-5Plateau Phase D-5SYBR Green Chemistry D-3PCR stepfill reservoirs, checking after centrifuging 4-18fill reservoirs, trimming after sealing 4-21general practices 4-6placing in bucket in centrifuge 4-17TaqMan Low Density Array, centrifuging 4-16TaqMan Low Density Array, loading 4-14TaqMan Low Density Array, placing inbucket 4-16TaqMan Low Density Array, sealing 4-19persisted dataanalysis sessions 1-28plate documents1-28studies 1-28pipetting errors 8-6pipettors, using 8-6plate adapter, changing 7-12plate document informationapplying to a plate document 3-28plate documents 3-9about 2-12adding to the plate queue 4-35, 4-40applying detector tasks 3-16applying sample names 3-28assigning standard quantities 3-17closing 2-18configuring document information 3-28copying detectors 3-13copying markers 3-15creating 2-13, 3-9creating from a template 3-26, 4-36exporting 2-14importing setup data 2-16, A-2methods, See methodsopening 2-19programming methods 3-19removing from the plate queue 4-40running batches 4-39running individually 3-29saving 2-17saving as a single plate file 4-24saving as a template file 3-24saving to the database 4-24setting Sample Volume 3-22setting the passive reference 3-18plate gridabout 2-29selecting wells 2-24viewing well information 2-23zooming 2-26Plate Handler 1-8, 7-36adjustment knob 7-36aligning 7-41 to 7-48cleaning the finger pads 7-52gripper 7-36plate stack positions 7-36Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideIndex-5DRAFTSeptember 1, 2004 11:38 am, 4351684AIX.fm