Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 6Analyzing Real-Time DataSetting the Baseline and Threshold ValuesSetting theBaseline andThreshold Valuesfor the RunIf you choose to set the baseline and threshold values manually for any detector in thestudy, you need to perform the following procedure for each detector.1. Select the Results tab.2. If setting the baseline and threshold for a:Absolute Quantification Plate DocumentSelect all wells of the plate by clicking the upper-left corner of the plategrid.ButtonRelative Quantification Multiple Plate Documenta. Select a plate in the Plates field to view, then select all wells of the plate byclicking the upper-left corner of the plate grid.b. Repeat step a to select all wells of every plate in the study.Click here to select allwells of the plate gridSelect a plate here3. In the Detector drop-down list in the Amplification plot, select a detector forwhich you want to set the baseline and threshold values.The software displays the data for the selected wells within the Amplification plot.4. In the Plot drop-down list, select ∆R n vs. Cycle.5. Double-click the Amplification plot, or click .6-46 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Real-Time.fm