Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 7Maintaining the InstrumentPreparing theVerification Plateor TGF-β CardPreparing a TaqMan RNase P Instrument Verification Plate1. Remove the TaqMan RNase P Instrument Verification Plate from the freezerand allow it to thaw to room temperature.2. Briefly centrifuge the TaqMan RNase P Instrument Verification Plate.3. Continue with “Preparing the Plate Document” on page 7-33.Preparing a TGF-β Card4. Remove the TGF-β card from the refrigerator and allow it to warm to roomtemperature.5. In a sterile 2-mL tube, prepare the following PCR reaction mixture:ReagentVolume (µL)TaqMan 2✕ Universal PCR Master Mix 500Human cDNA 50RNase/DNase-free water 450Total Volume 10006. Tap the tube gently to mix.7. Pipette 100 µL of the reaction mix into each of the eight fill reservoirs.8. Continue with “Preparing the Plate Document” on page 7-33.7-32 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm