Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

After the RunAfter the RunUser AccessRequirementEjecting a Plate(Opening andClosing theInstrument Tray)If using the SDS Enterprise Database, you must belong to the Scientist orAdministrator User Group to save a plate document.View the instrument status icon to determine the plate document to use to eject the plate.Instrument StatusConnected toplatenameAction1. In the SDS software, select Window >(the plate documentconnected to the instrument).2. In the plate document, select the Instrument tab3. Select the Plate-Read or Real-Time tab.4. Click .Disconnected1. In the SDS software, click (or select File > New).2. Click .3. Select the Instrument tab.4. Select the Plate-Read or Real-Time tab.5. Click .Saving theRun DataAfter the run, you must either save the results to the SDS Enterprise Database or savethe run data to the SDS 7900HT Document (*.sds).To save the run data to the SDS 7900HT Document:1. In the SDS software, select File > Save.To save the run data to the SDS Enterprise Database:IMPORTANT! If you saved the plate document to the database prior to running theplate, the SDS software stored the run data as a temporary file in the /temp directory. Ifyou closed your plate document at the end of the run without saving it, open the tempdirectory and rename the temporary file to *.sds and you will be able to open the file.1. Select File > Save Document to Database:2. In the Comment field of the Save Document to Database dialog box, enter abrief description of the plate document (up to 255-characters).Note: If saving a plate document file to the database, you will loose the filename unless you enter it into the Comments field.3. Click .4. In the Saved Document dialog box, click .Analyzing theRun DataSection 5.1 Allelic Discrimination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Section 6.1 Absolute Quantification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5Section 6.2 Relative Quantification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-15Section 6.3 Dissociation Curve Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-37Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 4-29DRAFTSeptember 1, 2004 11:39 am, CH_Basic.fm