Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Step 1 – Creating a Plate DocumentStep 1 – Creating a Plate DocumentUser AccessRequirementAbout ABI PRISMPlate DocumentsCreating a PlateDocumentIf using the SDS Enterprise Database, you must belong to the Scientist orAdministrator User Group to create plate documents.Every plate run on the 7900HT instrument requires the creation of a plate documentwithin the SDS software. Each plate document is a virtual representation of aspecific consumable (that is, a reaction plate or Low Density Array) containingsamples and reagents for use on the 7900HT instrument.Plate documents contain the following information:• Detector information and arrangement on the plate• Marker information and arrangement on the plate (allelic discrimination only)• Sample information and arrangement on the plate• Method parameters for the run (absolute and relative quantification)1. If not already open, start the SDS software as explained on page 3-8.2. Click (or select File > New).3. Configure the New Document dialog box with settings for the run:• Assay drop-down list – Select the type of assay appropriate for your plate.• Container drop-down list – Select the type of consumable you intend to run.• Template drop-down list – Select Blank Template.• Barcode field – Do one of the following:If you are creating a:– Plate document to run a single plate, scan or enter the bar code for the plate.– Plate Document Template for creating multiple plates, leave the fieldblank.IMPORTANT! The SDS Enterprise Database does not support the creation oftwo plate documents of different run types with the same bar code.Note: If performing a dissociation curve experiment, select AbsoluteQuantification from the Assay drop-down list.Assay drop-down listContainer drop-down listTemplate drop-down listBarcode field4. Click .Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 3-9DRAFTSeptember 1, 2004 11:39 am, CH_Run.fm