Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Performing a Pure Dye RunAfter a run, the SDS software receives run data in the form of a raw spectra signal foreach reading. To make sense of the raw data, the software must determine thecontribution of each fluorescent dye used in the sample through a process calledmulticomponenting. The software accomplishes the separation by comparing the rawspectra with a set of pure dye standards contained in a calibration file. When a platedocument is saved after analysis, the software stores the pure spectra informationwith the collected fluorescent data for that experiment.MaterialsRequired384- or 96-Well Plate• Appropriate Spectral Calibration Kit:– Sequence Detection Systems 384-Well Spectral Calibration Kit– ABIPRISM 7900HT Sequence Detection Systems 96-Well SpectralCalibration Kit– 7900HT System Fast 96-Well Spectral Calibration KitNote: Both the standard 96-well and Fast 96-Well Spectral Calibration Kitscontain two pure dye plates.• Product Insert• Centrifuge, with plate adapterMicrofluidic Pure Dye Cards• Microfluidic card spectral calibration reagents• Centrifuge• Microfluidic card sealerApplied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 7-21DRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm