Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Preparing a Run 33In This Chapter Notes for Database Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4Workflow Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5Quick Review: Powering On the 7900HT Instrument. . . . . . . . . . . . . . . . . . . . . . . 3-8Step 1 – Creating a Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9Step 2 – Applying Detectors and Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11Step 3 – Configuring the Plate Document with Tasks . . . . . . . . . . . . . . . . . . . . . 3-16Step 4 – Setting the Passive Reference and Omitting Wells . . . . . . . . . . . . . . . . . 3-18Step 5 – Programming the Plate Document Method. . . . . . . . . . . . . . . . . . . . . . . 3-19Step 6 – Saving the Plate Document as a Template. . . . . . . . . . . . . . . . . . . . . . . . 3-24Step 7 – Creating a Plate Document from the Template . . . . . . . . . . . . . . . . . . . . 3-26Step 8 – Applying Sample and Plate Information. . . . . . . . . . . . . . . . . . . . . . . . . 3-28Step 9 – Running the Plate on the 7900HT Instrument. . . . . . . . . . . . . . . . . . . . . 3-29Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 3-1DRAFTSeptember 1, 2004 11:39 am, CH_Run.fm