Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 4Operating the InstrumentBefore You BeginPre-RunChecklistThe following tasks must be complete to run plates on the 7900HT instrument. Seethe associated page number for details on each procedure.Done Check See PageA background run has been performed in the last month 7-16A pure dye run has been performed in the last 6 months 7-20The instrument tray does not contain a plateIMPORTANT! The instrument tray must be empty to begin a run. Ifthe instrument tray contains a plate, you must eject and remove it.4-44If using an Automation Accessory, also check the following…The output stack does not contain plates. –WorkflowOverview1. Prepare your experiments for use on the 7900HT instrument:– Prepare optical plate(s) (see page 4-8).– Prepare TaqMan ® Low Density Array(s) (see page 4-10).2. Prepare and run the 7900HT instrument:– Stand-alone Operation (see page 4-23).– Automated Operation using the SDS Enterprise Database (see page 4-32).– Automated Operation without the database (see page 4-34).3. Analyze the run data.– Allelic Discrimination Analysis (see page 5-5).– Absolute Quantification Analysis (see page 6-5).– Relative Quantification Analysis (see page 6-15).– Dissociation Curve Analysis (see page 6-37).4-4 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Basic.fm