Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Exporting Plate Document Data4. In the Export drop-down list, select the type of data you would like to export.• Background Spectra – The exported file contains the fluorescence readingsfor each well from the background component used to analyze the run• Clipped – The exported file contains the average R n and ∆R n of the lastthree data points collected during the extension phase of each cycle repetitionfor each well• Dissociation Curve – The exported file contains:Temperature Data – For each well in use on the plate, the file displays thecalculated temperature of the wells during each data collection reading of thetemperature ramp.Raw Data – For each well in use on the plate, the file displays the R n of thewell during each data collection reading of the temperature ramp.Derivative Data – For each well in use on the plate, the file displays the firstderivative data during each data collection reading of the temperature ramp.• Multicomponent – The exported file contains:– Amounts the calculated dye components in a single well throughout allstages of the PCR that were labeled with data collection icons– Pure spectra component data– Calculated inverse matrix– Singular values of the inverse matrix• Pure Spectra – The exported file contains the fluorescence readings for eachwell from the pure spectra calibration component used to analyze the run• Raw Spectra – The exported file contains the unmodified fluorescencereadings taken for each spectral bin during the course of the run• When exported, the software creates a directory and saves each the rawspectra data for each well in a separate *.txt file.• Results Table – The exported file contains the contents of the table pane ofan analyzed plate documentNote: The contents of the exported data vary depending on the type of platedocument.• Setup Table – The exported file contains the contents of the table pane of aplate document before analysis The contents of the exported data variesdepending on the type of plate document used to produce it. See page A-4 fora detailed description of the Setup Table file.5. To export data from:• All wells of the plate document – Select the All Wells radio button.• Selected wells of the plate grid – Select the Selected Wells radio button.6. In the files of type drop-down list, select the appropriate format for the exporteddata.7. Click the File name text box, and enter a name for the exported file.8. Click .The software saves the exported data to the designated location.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide A-17DRAFTSeptember 1, 2004 11:39 am, App_SWReference.fm