Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Appendix ASoftware ReferenceWell-Detector Information10. Well-Detector List HeaderElement numbers 10 and 11 define the contents of the wells on the plate in terms ofdetectors. The well-marker information consists of two sections: the Well-DetectorList Header and the Well-Detector Definition List.DescriptionThis line contains the column headings for the well-marker information section ofthe setup table file that make the file easier to edit using a program such as MicrosoftExcel.Format [ Well Sample Name Detector Task Quantity ] {... Detector Task Quantity } [ ]ExampleExample Code A-7Well Sample Name Detector Task Quantity Detector Task Quantity11. Well-Detector Definition ListDescriptionThis section defines the contents of the plate wells. The setup table file must contain adefinition for each well used on the plate. Each well definition list consists of one stringof characters terminated by a . The definition consists of three main functionaldivisions:• Well number – The first tab-delimited text block defines the number of the well onthe plate. Well numbers start at 1 for well A-1 (upper-left corner of the plate) andincreases from left to right and from top to bottom. The wells must be listed inorder (1,2,3,…).• Sample name – The second text block defines the name of the sample assigned tothe well.• Detector assignments – The remaining tab-delimited text blocks for the welldefinition define the detectors assigned to the well. Each detector is represented bythree text blocks that define the following information:– The name of the detector– The task assignment of the detector for the well (UNKN - Unknown, STND -Standard, NTC - No Template Control)– The quantity assignment of the detector for the well. (For wells containingstandards, assign the quantity for the standard sample in initial copy number. Forall other wells, assign the quantity value as 0.)To assign more than one detector to a well, repeat the detector definition text blocks foreach detector. There is no limit to the number of detectors that can appear in a well.IMPORTANT! All detectors that appear in this section must have been previouslydefined in the Detector Definitions section (elements 4–6).A-10 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, App_SWReference.fm