Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 3Preparing a RunAllelic DiscriminationDetectorAbsolute or RelativeQuantification Detector4. Click .The software saves the new detector and displays it in the detector list.Note: If using the SDS Enterprise Database, you cannot create a detector thatthat uses the same combination of detector name, reporter dye, and quencherdye as an existing detector.5. Repeat steps 2 through 4 to create detectors for all remaining assays on the plate.Note: Click the button for information on the Detector Manager dialog boxor to view the procedures for editing, deleting, or searching for detectors.6. Choose from the following:If constructing aplate document for…• absolute quantification• relative quantification• dissociation curve analysisallelic discriminationThen…copy the detector(s) to the plate document asexplained in the procedure on page 3-13.create and apply markers to the platedocument as explained on page 3-14.3-12 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Run.fm