Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

7900HT FAST Real-Time PCR SystemChapter 2Getting StartedPowering On the 7900HT InstrumentPowering On theInstrumentThe activation of the Applied Biosystems 7900HT Fast Real-Time PCR System issequential. You must activate each component in a specific order for the system toinitialize properly. If performed out of sequence, the components may not be able toestablish the necessary communication connections required for operation.Status lightsGR20097900HTFront view with RobotComputerpower button7900HT instrumentpower buttonGR2009Zymark Twister MicroplateHandler (power button in rear)Monitorpower buttonFigure 2-1 Power Buttons of the Applied Biosystems 7900HT Fast Real-TimePCR SystemIMPORTANT! Power on the power to the instrument and the Plate Handler at least10 min before use. When activated, the instrument heats the sample block cover to105 °C. If you start a run before the heated cover reaches 105 °C, the instrument willpause until it reaches the optimal temperature before commencing the run.1. Power on the monitor and computer.2. Power on the Zymark Twister Microplate Handler by pressing the power switchlocated on the back panel of the Plate Handler (see below).PHYSICAL HAZARD. Keep clear of the arm whenactivating the Plate Handler. Once activated, the arm automatically moves to itshome position.HI-POTA B C DPower switchRear Panel of the TwisterGR1728If operating normally, the Plate Handler moves the arm to the home position(over the output stack).3. Power on the 7900HT instrument by pressing the power button located belowthe status lights on the front of the instrument (see Figure 2-1).If operating normally, the 7900HT instrument will do the following on startup:• Emit a high-pitched tone signaling that the instrument initialized successfully.2-4 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:38 am, CH_GetStart.fm