Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

7900HT FAST Real-Time PCR SystemAligning the Plate HandlerAligning the Plate HandlerWhen to PerformPreparing theInstrument for theAlignmentPerform the following procedure if the Applied Biosystems 7900HT Fast Real-TimePCR System is moved or the Zymark Twister Microplate Handler becomesmisaligned.Symptoms that the Plate Handler is out of alignment include:• Excessive downward movement of the Plate Handler arm (the arm grinds whengrasping or releasing plates)• The Plate Handler arm collides with the plate stacks• The Plate Handler arm releases plates above the bottom of the plate stacks• Reaction plates tip or tilt when placed into the instrument tray by the arm1. Remove the covers for the fixed-position bar code reader and the underlyingplatform.GR20097900HTFront view with RobotGR2009Fixed-position bar code readerand underlying platform covers2. Loosen the three black thumbscrews on the platform connecting the 7900HTinstrument and the Plate Handler base.Black thumbscrews3. Move the instrument tray to the out position.a. Start the Automation Controller Software.If an error dialog box appears reading, ‘Machine calibration values arenot valid. Please refer to documentation for calibration process,’click .b. Click .The 7900HT instrument moves the instrument tray to the out position,perpendicular to the instrument.c. Select File > Exit.The software quits the Automation Controller Software.4. Select > Programs > Zymark Twister Plate Handler > Twister.The Zymark Twister Software starts.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 7-41DRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm