Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Running Plates Using the Automation Controller SoftwareRunning Plates Using the Automation Controller SoftwareUser AccessRequirementThere is no access requirement. All users can run plates that have been saved to theSDS Enterprise Database.Loading Plates onto the Plate HandlerGuidelinesObserve the following guidelines when loading plates onto the Plate Handler:• Before loading plates onto the Plate Handler, make sure that for each plate:– the associated plate document has been added to the plate queue or iscontained in the SDS Enterprise Database– the plate has been sealed using an optical adhesive cover.• Load the plates into the Plate Handler stacks in any order. The software readsthe bar code of each plate before it is run and matches the plate document andmethod with the plate. The Automation Controller Software can run batches ofup to 84 plates in a single session (21 plates/stack).• Orient the plates inside the stacks so that well A1 ( ) of each plate correspondsto the locations shown in Figure 4-11.• Do not place plates in the output stack (X). The Plate Handler arm uses theempty stack to store plates after it runs them.• If loading Low Density Array(s) onto the Plate Handler:– Load no more than 20 cards per stack.– Pack stacks of cards tightly. (The Plate Handler may fail to detect amisaligned card correctly when loading it.)X1423Zymark Twister MicroplateHandler (top view)Bar codeWell A1Figure 4-11Plate Positions of the Zymark Twister Microplate HandlerApplied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 4-41DRAFTSeptember 1, 2004 11:39 am, CH_Basic.fm