Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

7900HT FAST Real-Time PCR SystemManaging Sequence Detection System DataManaging Sequence Detection System DataSystemOperation andDataflowStandard Modesof OperationData management strategy is a crucial element of successfully integrating theApplied Biosystems 7900HT Fast Real-Time PCR System into a laboratoryworkflow. During a single 24-hour period of real-time operation, the 7900HTinstrument can produce more than 200 MB of data. To successfully manage theinformation produced by the 7900HT instrument, you should have a basicunderstanding of how data is collected, stored, and processed throughout theoperation of the instrument.The Applied Biosystems 7900HT Fast Real-Time PCR System has three standardmodes of operation depending on the accessories purchased with the base instrument• Stand-Alone Operation (see below)• Automated Operation (see page 1-18)• Database Operation (see page 1-18)Stand-Alone OperationThis mode uses the most basic configuration of the Applied Biosystems 7900HT FastReal-Time PCR System (Figure 1-11) where the 7900HT instrument is used without anyadditional components. In this configuration, a technician runs plates individually usingthe SDS software which stores all data on the computer hard drives.ComputerGR21797900HT raveninstrument frontSDS SoftwareApplied Biosystems 7900HTFast Real-Time PCR SystemInstrument Firmware12Plate DocumentCreatedPlate Document Usedto Run a PlateHard drive(s)3Thermal Cycling andSequence Detection5Raw Data Saved tothe Plate Document*.sdsfile*.sdtfile4Data CollectionSerialCable6Plate DocumentOpened and AnalyzedFigure 1-11Stand-Alone Operation of the 7900HT InstrumentApplied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 1-17DRAFTSeptember 1, 2004 11:38 am, CH_Overview.fm