Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 8TroubleshootingTaqMan Low Density ArrayLow Density ArrayTroubleshootingTableTable 8-6Troubleshooting the TaqMan ® Low Density ArrayObservation Possible Cause Recommended ActionAfter removing the LowDensity Array from itspackaging, the fillconsumable is damaged(creased, bent, or folded).After removing the LowDensity Array from itspackaging, the aluminumfoil backing is damaged(creased, bent, or folded).After removing the LowDensity Array from itspackaging, dust or otherparticulates settle on thereaction wells (optical sideof the Low Density Array).After removing the LowDensity Array from itspackaging, watercondenses on the reactionwells (optical side of theLow Density Array).N/AN/AN/AThe Low Density Array maynot have come to roomtemperature before beingremoved from itspackaging.If the fill consumable isdamaged, then the PCRreaction mixture might notflow into the reaction wellsduring centrifugation.Discard the Low DensityArray.If the aluminum foil backingis damaged, then the PCRreaction mixture might notflow into the reaction wellsduring centrifugation.Discard the Low DensityArray.Remove dust or particulatesby lightly tapping or blowingon the reaction wells.Room-temperature,pressurized nitrogen or anair blower may be used.IMPORTANT! Be sure toremove all dust andparticulates. The reactionwells (optical side of theLow Density Array) must befree of dust andparticulates, (especiallyfluorescent particulates)Remove condensation bylightly blowing on thereaction wells.Room-temperature,pressurized nitrogen or anair blower may be used.IMPORTANT! Be sure toremove all watercondensation. The exteriorsurface of the reaction wells(optical side of the LowDensity Array) must be freeof water condensation.8-18 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Trouble.fm