Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

SDS 7900HTDocument (*.sds)SDS 7900HTTemplateDocument (*.sdt)absolutequantification (AQ)alleleallelicdiscrimination (AD)ampliconAmplification plotanalysis sessionannealingApplicationProgram Interface(API)assay typeauto incrementvalueA file used by the SDS software to store plate documentation locally on a computerhard drive. A plate document is a virtual representation of a consumable (plate) usedto contain samples and reagents during a sequence detection run. During the run, thesoftware uses the plate document to record the operation of the instrument (thermalcycling and data collection parameters), organize and store the data gathered duringthe PCR process, store the of the run data, and record analysis and display options.Plate document templates have the same format as the SDS 7900HT Documents(*.sds) but do not store raw data or bar codes.The objective of an absolute quantification experiment is to accurately determine theabsolute quantity of a single nucleic acid target sequence within an unknown sample.The results of an absolute quantification experiment are reported in the same units ofmeasure as the standard used to make them.One of the variant forms of a gene at a particular locus or location on a chromosomeThe process by which two variants of a single nucleic acid sequence present in apopulation of individuals' DNA samples is determined. Single nucleotidepolymorphisms (SNPs) comprise the most common variants that are assayed usingTaqMan reagents.A PCR amplified section of DNAThe Amplification plot displays data from real-time PCR for gene expression assaysafter signal normalization and multi-component analysis. The Amplification plotappears only in plate documents containing analyzed data from real-time runs. It isa 2D plot of normalized reporter fluorescence (for ∆R n ) values versus the cyclenumber. The ∆R n versus cycle Amplification plot is used for calculation thresholdcycle (C T ).A set of analysis results derived from a single raw data set associated with a singleplate document analyzed by the SDS software. The analysis session is persisted in thedatabase and can be retrieved by the SDS software. Multiple analysis sessions can becreated from the same plate document, however each analysis session must have aunique name. An analysis session does not have to be associated with a Study;however, if it has such a relationship, it can be associated with at most one Study atany given time.Binding a probe to the DNA or cDNA during the PCR processA set of routines, protocols, and tools for building software applications. A good APImakes it easier for a programmer to develop a program by providing all the buildingblocks necessary to link the elements of the various subcomponents of the system.With reference to the SDS 2.2 Enterprise Option software, an assay type is one of thefollowing: allelic discrimination, absolute quantification, or relative quantification.Method setting that increases or decreases the value for any PCR set-point parameter(time or temperature) by a fixed amount every cycle.Glossary-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, Glossary.fm