Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

elative quantification B-6automatic outlier removal 6-19Automation Accessorycomponents 7-36Plate Handler, See Plate HandlerSee fixed-position bar code readerAutomation Controller Softwareabout 4-39adding plates to the plate queue 4-40configuring for operation 4-42ejecting a plate 4-44launching 4-39monitoring instrument progress 4-44removing plates from the plate queue 4-40starting the plate queue 4-43stopping the plate queue 4-44Bbackground run 7-16 to 7-19about 7-16constructing a background plate 7-17creating a plate document 7-17extracting 7-19preparing a background plate 7-17troubleshooting 8-9when to perform 7-16bar code informationentering into a plate document 2-27, 3-28bar code readersfixed position, See fixed-position bar code readerhand held, See hand-held bar code readerbars, in the Gene Expression Profilesymbols 6-31baselineabout D-6configuring value for automatic analysis 6-11Block readout (from the Real Time tab) 4-27Bucket Type, setting on centrifuge 4-13Ccalibrating the 7900HT instrumentadjusting the plate-sensor switch 7-37aligning the fixed-position bar code reader 7-49aligning the Plate Handler 7-41performing background run 7-16performing pure dye (spectral) run 7-20calibrator sample 6-20Centrifuge systembucket requirements 4-16Bucket Type, setting 4-13components 4-12installation and operation 4-13placing bucket in centrifuge 4-17requirements 4-12TaqMan Low Density Array, placing inbucket 4-16Centrifuging, TaqMan Low Density Array 4-16changinggripper finger pads 7-52pane, view, and plot sizes 2-21sample block module 7-6checklistsabsolute quantification 6-9allelic discrimination 5-10dissociation curve 6-22, 6-40relative quantification 6-22chemistry5´ Nuclease (TaqMan) D-2non-optimized 8-6SYBR Green D-3troubleshooting 8-5 to 8-8cleaninggripper finger pads 7-52sample block wells 7-15closinginstrument tray (Automation ControllerSoftware) 4-44instrument tray (SDS software) 4-29plate documents 2-18Zymark Twister Software 7-48commentsadding to a detector 3-11adding to a plate document 3-28Comparative C T Methodabout 6-4, D-8formula derivation D-8Componentscentrifuge system 4-12TaqMan Low Density Array 4-10computerabout 1-6hard drive partitions 1-6maintaining 7-54minimum system requirements 1-6troubleshooting 8-14turning ON 2-4configuring the 7900HT instrument for 9600emulation 3-21consumables384-Well Optical Reaction Plates C-396-Well Optical Reaction Plates C-4improper or damaged plastics 8-8Optical 96-Well Fast Thermal Cycling Plates C-4Optical Adhesive Covers C-3Optical Cap Strips C-3writing on reaction plates 8-7Contaminationgenomic DNA 4-11preventing 4-6contaminationdecontaminating the sample block 7-14Index-2Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:38 am, 4351684AIX.fm