Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

IndexSymbols– 6-31↑ 6-33Numerics7900HT instrument, See instrument9600 emulation mode 3-21AABI PRISM 7700 Sequence Detection System,emulating 3-21ABI PRISM Sealer, see Sealerabsolute quantificationabout 6-6 to 6-7analyzing data 6-9 to 6-13assay development guidelines B-6procedure checklist 6-9selecting and preparing standards B-6troubleshooting 8-12addingbar code to a plate document 2-27, 3-28custom dyes to the pure dye set 7-27detector tasks to a plate document 3-16detectors to a plate document 3-13markers to a plate document 3-15plate documents to the plate queue 4-35, 4-40sample names to a plate document 3-28adjustinganalysis options for absolute quantification 6-11display settings 3-24method step parameters 3-21plate-sensor switch 7-37 to 7-40adjustment knob 7-36air bubbles 8-6aligningfixed-position bar code reader 7-49 to 7-51Plate Handler 7-41 to 7-48allele callsabout 5-15calling 5-14scrutinizing 5-16allelic discriminationabout 5-6 to 5-8analyzing run data 5-10 to 5-18assay development guidelines B-5maximizing throughput 3-4procedure checklist 5-10thermal cycling on the 7900HT instrument 3-20troubleshooting 8-13Allelic Discrimination Plotabout 5-13calling alleles 5-14datapoint cluster variations 5-8exporting as a graphic file A-16exporting data as a text file A-16genotypic segregation 5-8outliers 5-8scrutinizing allele calls 5-16amplificationbeyond reproducible limits in multi-reporterexperiments 6-19amplification curveabout D-4Geometric (Exponential) Phase D-4Linear Phase D-5Plateau Phase D-5Amplification Plotexporting as a graphic file A-16exporting data as a text file A-16Analysis 6-22analysis sessions, about 1-28analyzingabsolute quantification data 6-9 to 6-13allelic discrimination data 5-10 to 5-18background data 7-19dissociation curve data 6-40 to 6-44pure dye data 7-25relative quantification data 6-22 to 6-35applyingdetector tasks 3-16detectors to a plate document 3-13markers to a plate document 3-15sample names 3-28architecturedatabase LAN setup 1-23database WAN setup 1-24Archiver 1-26archiving files 7-54arrows, sample bars 6-31assay development guidelinesabsolute quantification B-2 to B-4, B-6allelic discrimination B-2 to B-5Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideIndex-1DRAFTSeptember 1, 2004 11:38 am, 4351684AIX.fm