Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Software and 7900HT InstrumentTable 8-4Troubleshooting Software and Computer ProblemsObservation Possible Cause Recommended ActionCommunication errorThermal cycler errorsAutomation ControllerSoftware cannot find a platedocument fileDialog box does not respondto mouse clicks or keystrokesCables are connectedincorrectlySample block modulenot fully engagedFile not in correctlocationJava Runtime ErrorCheck cable connections andCOM port setup. See“Instrument Connections” onpage 1-10.Reseat the sample blockmodule as explained“Replacing the Sample Block”on page 7-6.Remove file entry from platequeue and add the file to theplate queue again.Click the close box of the dialogbox to close it.Run will not start No calibration file Perform background and puredye runs.No background data inSee “Performing a Backgroundcalibration fileRun” on page 7-16 and(background run has“Performing a Pure Dye Run”not been performed)on page 7-20.No pure dye data incalibration file(pure dye run has notbeen performed)Calibration file doesnot contain pure dyedata for a dye used onthe plate documentCalibration file wascreated on anotherinstrumentDisk drive containingthe plate documenthas less than 50 MB offree spaceHeated cover cannotreach runningtemperature becauseno plate loadedCheck the capacity of thedestination drive. If less than50 MB of free space remains,remove or archive existing datafiles (see page 7-54).Open the instrument tray andcheck that the instrumentcontains a plate.Instrument traycontains a plateOutput stack containsa plate or platesRemove all plates from theoutput stack of the PlateHandler before starting thequeue.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 8-15DRAFTSeptember 1, 2004 11:39 am, CH_Trouble.fm