Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Running a Single Plate (Using the SDS Software)MonitoringInstrumentProgressThe SDS software displays instrument status and run progress in the Real-Time tab(real-time runs) or the Plate Read tab (end-point runs) of the respective platedocument. Figure 4-10 shows examples of the tabs during operation of the 7900HTinstrument.Real-Time Tab(Real-Time Runs)Plate Read Tab(End-Point Runs)Figure 4-10Real-Time and Plate Read Tabs of the SDS Software• Status – Displays the condition of the 7900HT instrument• Time Remaining – Displays the calculated time remaining in the runTemperature group box (Real-Time Plate Documents Only)• Block – Displays the actual temperature of the sample block module• Cover – Displays the actual temperature of the heated cover• Sample – Displays the calculated temperature of the samplesCycle group box (Real-Time Plate Documents Only)• Rep – Displays the current cycle repetition• Stage – Displays the current stage of the thermal cycling• State – Displays the current condition of the cycle stage• Step – Displays the current step being run• Time – Displays the calculated time remaining in the current stepData Collection Stamp group box (End-Point Plate Documents Only)• Pre – Displays the date that the instrument performed the pre-read.• Post – Displays the date that the instrument performed the post-read.Note: A ‘pre’ read is a plate read performed before a plate has undergone thermalcycling.Note: A ‘post’ read is a plate read performed after a plate has undergone thermalcycling.Note: For more information on the elements of the Real-Time and Plate-Read tabs,click and see the Sequence Detection Systems Software Online Help.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 4-27DRAFTSeptember 1, 2004 11:39 am, CH_Basic.fm