Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 7Maintaining the Instrumentb. If the data set contains an outlying peak, eliminate it by clicking the checkbox of the associated well.Note: Dye spectra are generally acceptable if they peak at the same location astheir group but diverge slightly at other wavelengths.c. Click when finished.d. Repeat steps a to c for all remaining wells until prompted with a messagereporting the extraction of the pure dyes.The software extracts the pure spectra and stores the data as a component of thecalibration file.3. Click (or select File > Save).The software saves the plate document.4. Select File > Close.The software closes the plate document.5. If performing spectral calibration of a:• Standard 384-Well Block – The pure dye calibration is complete.• Standard 96-Well Block – Run the second Pure Dye plate by repeating theprocedures for:– “Preparing a Plate Document for a Pure Dye Plate or Card” on page 7-22– “Running the Prepared Pure Dye Plate or Card” on page 7-24– “Analyzing the Pure Dye Run” on page 7-25• 7900HT System TaqMan Low Density Array Upgrade – Run the secondand third microfluidic pure dye cards containing the VIC and ROX dyes byrepeating the procedures for:– “Preparing a Plate Document for a Pure Dye Plate or Card” on page 7-22– “Running the Prepared Pure Dye Plate or Card” on page 7-24– “Analyzing the Pure Dye Run” on page 7-257-26 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm