Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 8TroubleshootingTroubleshooting TableOverviewThe following table is designed to help you troubleshoot most of the problems youmay encounter while using the Applied Biosystems 7900HT Fast Real-Time PCRSystem.The information in the table is arranged by category as follows:• Chemistry problems• Run problems• Instrument and Automation Accessory ProblemsEach category contains subcategories, followed by a brief description of thesymptoms you might encounter.To use this table, look for the category and the symptom you are experiencing. Thepage number in the right-hand column corresponds to a description of the possiblecause(s) and recommended action(s) for that particular problem.Table 8-1Troubleshooting TableCategory Symptom PageChemistry and Run ProblemsChemistry Low Precision 8-5IrreproducibilitySoftware InstallationSDS SoftwareRun ProblemsUnable to finish installation(Install program appears to b e frozen)Installation Interrupted8-5Background Runs Software will not extract background data 8-9Background is too high (greater than 2500)Pure Dye Runs Software will not extract pure dye data 8-11Raw data from pure dye run appears strangeSignals plateau (saturation)Signal is too low (< 10,000 FSU)More than two outliers per dye in a single rowReal-Time Runs (Quantitative PCR and Dissociation Curves) 8-12End-Point Runs (Allelic Discrimination) 8-138-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Trouble.fm