Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Calling and Scrutinizing Allelic Discrimination Data4. Select Call > Allele X.Select Allele XThe software automatically labels the samples and wells with the Allele X call.Allele Xhomozygotes5. Repeat steps 3 through 4 to apply calls to the rest of the samples within the plot.Call Symbol DefinitionAllele X ● Homozygous for the allele displayed on the associatedaxis of the Allelic Discrimination Plot.Allele Y●Both ● Heterozygous (Alleles X and Y)NTC ■ No Template ControlUndetermined ✕ Unknown (Unlabeled)Note: You can adjust the appearance of the allelic discrimination plot or thedata points it contains using the Display Settings dialog box. See the SequenceDetection Systems Software Online Help for more information.Allele Y homozygotesAllele X/Allele YheterozygotesAllele X homozygotesNo amplification6. If evaluating for multiple markers, do the following:a. In the Marker drop-down list, select a different marker.b. Repeat steps 2 through 5 for the new marker.c. Repeat steps a and b until the alleles for each marker have been called.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 5-15DRAFTSeptember 1, 2004 11:39 am, CH_End-Point.fm