Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Verifying Instrument PerformancePreparing thePlate Document1. Start the SDS software.2. Click (or select File > New).3. Configure the New Document dialog box.Assay – Absolute QuantificationContainer – Select the appropriate plate format.Template – If running a:• TaqMan ® RNase P 384-Well Instrument Verification Plate, select384 Well RNaseP Install Plate.sdt.• TaqMan ® RNase P Instrument Verification Plate (standard 96-well plate),select 96 Well RNaseP Install Plate.sdt.• TaqMan ® RNase P Fast 96-Well Instrument Verification Plate, select96 Well RNaseP Install Plate.sdt.• TGF-β card, select rnase p card template.sdt.Note: If no plate document templates are available, construct a Pure Dye platedocument using the product insert from the Spectral Calibration Kit and theprocedure on page 7-28.4. If desired, enter the bar code information into the plate document:a. Click the Barcode text field.b. Remove the TaqMan RNase P Instrument Verification Plate or TGF-β cardfrom the packaging and scan its bar code using the hand-held bar codereader.5. Click . The software creates a plate document.Note: Do not modify the plate document. The plate document template ispre-programmed with detector and method information for the run.6. Save the plate document:a. Click (or select File > Save).b. In the Barcode field of the Save dialog box do one of the following:– Enter a name or bar code number for the plate, then click .– Using the hand-held bar code reader, scan the bar code number.c. Select Files of type > SDS 7900HT Document (*.sds).d. Click .The software saves the plate document. The software is now configured for theRNase P run.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 7-33DRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm