Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

single-plex TaqMan reagents 4-11TaqMan primers and probes 4-10trimming after sealing 4-21TaqMan reagents, description 4-10TaqMan RNase P Instrument Verification Platesabout 7-30analyzing 7-34kits C-5preparing a plate document 7-33running 7-30, 7-32Targets, mRNA, number per well 4-10tasks, See detector taskstemplatesabout 2-12creating a single plate document 3-26creating multiple plate documents 4-36saving as 3-24thermal cycler block, See sample block modulethermal cycling protocol, See methodsthresholdabout D-6configuring for automatic analysis 6-11improper setting 8-5threshold cyclecalculation D-6relationship to PCR product D-7Time readout (from the Real Time tab) 4-27Time Remaining readout (from the Real Timetab) 4-27T m , See melting temperaturetroubleshooting 8-2 to 8-207900HT instrument 8-14background runs 8-9chemistry problems 8-5 to 8-8computer 8-14end-point runs 8-13fixed-position bar code reader 8-17, 8-20pure dye runs 8-11real-time runs 8-12SDS software 8-14Zymark Twister Microplate Handler 8-17, 8-20turning ON the Applied Biosystems 7900HT Fast Real-Time PCR System 2-4Wresizing 2-21, 2-22showing 2-21warranty statement E-1well inspector 2-31YY Inter readout (from the Standard Curve Plot) 6-13Zzoomingallelic discrimination plot 5-14plate grid wells 2-26Zymark Twister Microplate HandlerSee Plate HandlerZymark Twister Softwareabout 1-14aligning the Plate Handler 7-41 to 7-48closing 7-48launchingUupgradingoperating system software 7-55SDS software 7-55Vviewing, well information 2-23viewshiding 2-21maximizing/minimizing 2-22Index-8Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:38 am, 4351684AIX.fm