Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

templatethresholdthreshold cycle (C T )T m(1) [SDS software: SDS 7900HT Template Document or plate document template] Apattern for creating an SDS 7900HT Document (plate document). The filenameextension for a plate document template is *.sdt. The plate document templatefacilitates creation of plate documents in the SDS software. It is typically a startingpoint for batch document setup. The binary representation of the plate documenttemplate is persisted in the database, allowing the plate document template to beretrieved and then populated with data and saved as an SDS 7900HT Document.(2) [Chemical] the DNA sample that is being amplified during PCR.An arbitrary level of normalized reporter signal (R n ) for C T determination in real-timeassays. The level is set to be above the baseline and sufficiently low to be within theexponential growth region of the amplification region of an amplification curve. Thethreshold is the line whose intersection with the Amplification plot defines the C T .For a given well, the threshold cycle (C T ) represents the PCR cycle at which the SDSsoftware first detects a noticeable increase in reporter fluorescence above a baselinesignal.(1)[Chemical] The temperature at which 50% of the DNA amplicons are in adouble-stranded configuration. (2) [Mathematical] The maximum value for the firstderivative curve of normalized reporter fluorescence (R n ).Glossary-8 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, Glossary.fm