Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 5Analyzing End-Point DataScrutinizing theAllele Calls1. Verify the calls for the NTC, Allele X, and Allele Y controls.a. In the plate grid, select the wells containing the No Template Controlsamples.The software highlights the datapoints within the allele plot.b. Check that the datapoints cluster in the expected position on the plot.c. If using positive controls, repeat steps a and b for the wells containing theAllele X, and Allele Y controls.Allele Y controlsshould cluster hereAllele X controlsshould cluster hereNo TemplateControls shouldcluster here2. Designate samples that did not cluster tightly as Undetermined.Samples that did not cluster tightly may:• Contain rare sequence variations• Contain sequence duplications• Contain contaminants• Be the result of errors in pipetting or sample preparationUndeterminedsamples5-16 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_End-Point.fm