Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Running Plates Using the Automation Controller SoftwareRunning the InstrumentStarting thePlate QueueAfter you have loaded all plate documents into the Plate Queue or saved them theSDS Enterprise Database, and you have configured the Instrument Control options,you can start the plate queue.To begin the plate queue, click from the Plate Queue tab.The Automation Controller Software does the following:1. The Zymark Twister Microplate Handler loads the first plate from Stack 1 andplaces it into plate tray for the 7900HT instrument.2. The fixed-position bar code reader scans the bar code for the plate.3. The Automation Controller Software does one of the following:• If linked to the SDS Enterprise Database, the software queries the databasefor the plate document with the bar code of the associated plate. Whenfound, the software automatically downloads the plate document.If the software does not find a plate document with a matching bar code inthe database, the software searches the contents of the plate queue asexplained below.• If not linked to a database, the software searches the plate queue for theplate document with the bar code of the associated plate. If found, theAutomation Controller Software loads the plate document informationfrom the file system for the run.4. The 7900HT instrument loads the instrument tray and runs the plate accordingto the parameters defined by the plate document file.Note: Before starting a real-time run, the instrument may pause (up to15 minutes) to heat the heated cover to the appropriate temperature.If using the SDS Enterprise Database, the Automation Controller Softwareautomatically conducts a primary analysis of each completed run and saves theresults to the database. If you specified a study or studies as explained in“Associating Session and Study Data” on page 4-33, then the softwareautomatically attaches the analyzed session data to the appropriate study.The following options are available during and after the completion of the run:To…See PageMonitor the progress of the run 4-44Stop the runIMPORTANT! If you must stop a run in-progress for any reason,carefully read the instructions on page 4-28 before halting the run.4-44Open the instrument tray (after the run) 4-44Analyze the run data after the run is complete 4-44Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 4-43DRAFTSeptember 1, 2004 11:39 am, CH_Basic.fm