Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 7Maintaining the Instrument4. Remove the plate and listen for the plate-sensor switch to disengage.Did you hear theswitch disengage?NoYesThen…1. Move the switch Down a few steps by turning the thumbwheel in the direction indicated on the arm.2. Replace the plate inside the gripper and listen for theswitch to engage:– If you do not hear the switch engage, then remove theplate and repeat steps 1 and 2 above.– If you hear the switch engage, remove the plate andcontinue to step a below.1. Move the switch Up by turning the thumb wheel one stepin the direction indicated on the arm.2. Replace the plate and listen for the switch to engage:– If you hear the switch engage, remove the plate andrepeat steps 1 and 2 above.– If you do not hear the switch engage, then you havesuccessfully identified the zero point of theplate-sensor switch.Note: At the zero point, one step of the thumb wheel in theDown direction causes the switch to engage.5. After the zero point is established, carefully turn the thumb wheel in the Downdirection the number of steps appropriate for your plate format as indicatedbelow:ConsumableABI PRISM 96-Well OpticalReaction PlateABI PRISM 384-Well OpticalReaction PlateTaqMan Low Density ArrayTurn the thumb wheel in the Down direction…20 steps15 steps15 stepsNote: If you lose count, begin again from step 4 and identify the zero point forthe switch.6. Test the adjustment as explained on page 7-39.7-38 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm