Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Analyzing the Study• Manual Ct – Instructs the software to use the baseline and threshold valuesspecified in the Analysis Settings dialog box to calculate C T s for the selecteddetectors.The software provides two methods for manually setting the baseline andthreshold values for the detector:– If you know the values that you want to use, enter them into the appropriatefields of the Analysis Settings dialog box.– If you want to set the baseline and threshold using the controls of theAmplification Plot tab, leave the fields empty and set the values later asdescribed on page 6-46.Replicate andOutlier Removalfor Study SettingsThe settings of this group box determine how the software identifies and removesoutlier data for all detectors in the study.Automatic OutlierRemoval check boxOmit replicates whose ∆C T