Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

PrefaceHow to Use This GuidePurpose of ThisGuideAudienceAssumptionsText ConventionsUser AttentionWordsSafety AlertWordsThe Applied Biosystems 7900HT Fast Real-Time PCR System and SDS EnterpriseDatabase User Guide describes how to prepare, maintain, and troubleshoot theApplied Biosystems 7900HT Fast Real-Time PCR System instrument, Zymark ®Twister Microplate Handler, and SDS Enterprise Database.This guide is written for technicians, scientists, and researchers of all skill levels whowill use and maintain 7900HT instruments with or without the SDS EnterpriseDatabase.This guide assumes that a Applied Biosystems technical representative has installedyour 7900HT instrument, Plate Handler, and SDS Enterprise Database and that youare familiar with:• Basic Microsoft ® Windows ® operations such as using the mouse, choosingcommands, working with windows, and using the hierarchical file system• Electronic storage devices (computer hard drives) and data files• Assay preparation and basic laboratory techniquesThis guide uses the following conventions:• Bold indicates user action. For example:Enter 0, then press Enter for each of the remaining fields.• Italics indicates new or important words and is used for emphasis. For example:Before performing a pure dye calibration, always perform a background run.• A right arrow bracket (>) separates successive commands you select from adrop-down or shortcut menu. For example:Select File > Open > Spot Set.Two user attention words appear in Applied Biosystems user documentation. Eachword implies a particular level of observation or action as described below:Note: Provides information that may be of interest or help but is not critical to theuse of the product.IMPORTANT! Provides information that is necessary for proper instrumentoperation, accurate chemistry kit use, or safe use of a chemical.Safety alert words also appear in user documentation. For more information,see “Safety Alert Words” on page xiv.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:38 am, Preface.fmxi