Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Determining T m Values for the Analyzed Run• T m display and slider – The SDS software displays the T m below the green slider(see below).T mThere are two definitions for the T m value:– The chemical definition is the temperature at which 50% of the DNA is in adouble-stranded configuration.– The mathematical definition is the maximum value for the first derivativecurve within a specific peak.• Plot drop-down list – Chooses the data displayed within the plot based on thederivative calculation. The list offers the following selections:– Raw – When selected, this option plots the normalized reporter fluorescencedata (R n ) on a graph of fluorescence vs. temperature (left-most plot inFigure 6-16).– Derivative – When selected, this option plots derivative data (R n´) on a graphof the derivative vs. temperature (right-most plot in Figure 6-16). Thederivative data is the negative of the rate of change in fluorescence as afunction of temperature.Figure 6-16 shows the plots accessible from the Plot drop-down list.Raw PlotDerivative PlotFigure 6-16Plots of the Dissociation Curve Tab• Detector drop-down list – Chooses the data displayed within the plot based ondetector name.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 6-43DRAFTSeptember 1, 2004 11:39 am, CH_Real-Time.fm