Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

R nrunsessionsingle nucleotidepolymorphism(SNP)singleplexSNPspectral binspectral calibrationspectral crosstalkstackstudytaskThe normalized reporter signal is the cycle-by-cycle ratio of the fluorescence of thereporter dye and the fluorescence of the passive reference dye in a given well. DuringPCR, R n increases as the amplification copy number increases until the reactionapproaches a plateau. When using the instrument in its Plate Read mode (end pointanalysis), the fluorescence signal is read at a single point in time after the completionof PCR rather than at intervals during the course of PCR.A run describes a collection of fluorescence data carried out by the 7900 instrumentduring and/or after cycling of samples in an optical plate or TaqMan ® Low DensityArray.See Analysis Session.A single base pair of DNA in the genome that differs between individuals.A PCR reaction with a single fluorescent detector per well.See single nucleotide polymorphism.A portion of the complete range of the wave length measured by the 7900HTinstrument (approximately 500-660 nm).Spectral calibration occurs from pure dye results obtained from a pure dye run. Thesoftware stores the spectral information for the pure dye standards within a calibrationfile located in the SDS directory. After a run, the software extracts each componentdye from the collected data in the pure spectra run file. Because the age and use of theinstrument components can affect pure spectra readings, Applied Biosystemsrecommends updating the pure spectra run file once or twice annually depending oninstrument use.The fluorescent contribution of one dye signal to another caused by the overlap of theirspectra.The physical container of a 7900HT instrument into which well plates can be stacked.There are 5 stacks in all. Four of the five consecutively numbered stacks are used tohold the sample plates while the fifth is used by the Plate Handler to hold the first setof analyzed plates. Maximally 20 plates can be placed in each stack. The instrumentcan process maximally eighty plates in one batch.A study in the SDS Enterprise Database option is a named collection of zero or moreAD or RQ analysis sessions. A study in the SDS software main application is an '.sdm'plate document file that contains one or more '.sds' relative quantification platedocument files.A setting applied to the detectors within a well of a plate document that determineshow the data collected from the well during analysis are used. The task selectionsavailable differ depending on the plate's assay type.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide Glossary-7DRAFTSeptember 1, 2004 11:39 am, Glossary.fm