Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Viewing ResultsViewing ResultsViewing theStandard CurveThe software displays the standard curve generated from the run data within theResults tab of the plate document. The standard curve plot displays the unknownsamples on a graph of C T (threshold cycle) vs. Quantity (LogN). Figure 6-5illustrates the components of the standard curve plot.Help buttonDetector drop-down listLegend boxHide Unknowns buttonStandard Curve boxStandard curve plotFigure 6-5Components of the Standard Curve Plot• – Starts the Sequence Detection Systems Software Online Help.• Detector menu – Toggles the data displayed within the plot based on detectorname.• Legend box – Displays a symbol key for the datapoints appearing in the plot.• Hide Unknowns button – Toggles the presence of data from unknown samplesin the plot.• Standard Curve box – Contains the following statistical data describing thestandard curve.– Slope – The slope of the standard curve. The slope of the standard curve isuseful for assessing the efficiency of the assay. At 100% efficiency, a reactionshould achieve a slope of −3.33 since every 10-fold difference in quantitytranslates to a difference of 3.33 C T s.– Y-Inter – The Y-axis intercept of the standard curve.– R2 – The R square value for the standard curve that describes the correlationbetween threshold cycles (C T ) and the log of the quantity value for thesamples that comprise the standard curve plot. The calculation yields a valuebetween 1 and 0, where values closer to 1 indicate better correlation betweenC T and the log of the quantity value.Note: The software calculates the R square value by taking the square of thePearson Coefficient of Correlation (also known as the r value) calculated for thedata points that comprise the plot. The software calculates the R2 value only forthe standards that make up the curve.• Standard curve plot – A scatterplot of datapoints from the absolutequantification run.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 6-13DRAFTSeptember 1, 2004 11:39 am, CH_Real-Time.fm