Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Calling and Scrutinizing Allelic Discrimination DataCalling and Scrutinizing Allelic Discrimination DataAbout the AllelicDiscriminationViewThe SDS software graphs the results of allelic discrimination runs on a scatterplotcontrasting reporter dye fluorescence. After signal normalization andmulticomponent analysis, the software graphs the normalized data from each well asa single data point on the plot. Figure 5-6 illustrates the components of the AllelicDiscrimination plot.Markerdrop-down listCalldrop-down listToolbarScatterplotFigure 5-6Components of the Allelic Discrimination Plot• Marker drop-down list – Determines the marker data that the software displayswithin the plot.• Call drop-down list – When a datapoint is selected, this menu allows you toassign an allele call to the datapoint within the scatterplot.• Toolbar – Contains the following tools for manipulating the plot.IconDescriptionSelects individual data points by clicking or groups of datapoints by clickingand dragging a box across a group of data points.Selects groups of datapoints by encircling them with the tool.Repositions the view within the plot by clicking and dragging the screen.Zooms the plot by clicking the mouse button within the plot or by clickingand dragging a section of the plot to view.Zooms out on the plot by clicking the mouse button within the plot.• Scatterplot – A scatterplot of data points from the run.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 5-13DRAFTSeptember 1, 2004 11:39 am, CH_End-Point.fm