Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Performing a Pure Dye Run• Fast 96-Well Pure Dye Run: select the plate document template matchingthe Pure Dye plate you intend to run.To run Plate 1 (containing FAM, JOE, NED, and ROX dyes), selectFast 96 Well Pure Dyes Plate 1.sdt.To run Plate 2 (containing SYBR, TAMRA, TET, and VIC dyes), selectFast 96 Well Pure Dyes Plate 2.sdt.• Microfluidic Pure Dye Run: leave the field blank.To run the microfluidic pure dye card containing FAM dye,select MFC FAM Pure Dyes.sdt.To run the microfluidic pure dye card containing ROX dye,select MFC ROX Pure Dyes.sdt.To run the microfluidic pure dye card containing VIC dye,select MFC VIC Pure Dyes.sdt.4. In the Barcode field, scan the bar code number using the hand-held bar codereader.5. Click . The software displays a plate document with the attributes for apure dye run.6. Save the Pure Dye plate document:a. Click (or select File > Save).b. Select Files of type > SDS 7900HT Document (*.sds).c. In the File name field, if running a:• Standard 384-Well Plate, enter PureDye_For example, the file name for a plate run on May 31, 2001 would be:PureDye_053101.• Standard 96-Well Plate, enter PureDye_Plate_For example, the file name for a Pure Dye Plate 1 run on May 31, 2001would be: PureDye_Plate1_053101.• Fast 96-Well Plate, enter FastPureDye_Plate_.sdsFor example, for a fast pure dye plate 1 run on May 31, 2001, type:FastPureDye_Plate1_053101.sds• Microfluidic pure dye card, enter _MFCFor example, the file name for a run performed on May 31, 2001 would be:FAM_053101_MFC.d. Click .The software saves the plate document.7. Prepare and run the pure dye plate or card as explained below.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 7-23DRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm