Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 3Preparing a RunCreating Markersfor AllelicDiscriminationAllelic discrimination plate documents feature the use of ‘markers’ to aid inorganizing and applying detectors based on the loci they target. A marker is a pairingof two detectors representing chemical assays designed to discriminate betweendifferent alleles of a common locus. The SDS software uses marker informationduring data analysis to organize and compare the processed run data.IMPORTANT! Allelic discrimination plate documents must contain at least onemarker.1. If the Detector Manager dialog box is open, click to close it.2. Select Tools > Marker Manager.3. In the Marker Manager dialog box, click .4. In the Enter name of new Marker field of the Add Marker dialog box, enter aname for the new marker, and click .The new marker appears in the Markers field.Note: If using the SDS Enterprise Database, you cannot create a marker thatthat uses the same combination of marker name and detectors as an existingmarker.5. Apply detectors to the new marker:a. In the Markers field, click the new marker to select it.The software highlights the selected marker.b. In the Detectors list, select the Use check boxes of the detectors that youwant to assign to the marker.The software highlights the selected detector.IMPORTANT! You cannot assign more than two detectors to a marker and thedetectors cannot use the same reporter dye.Detectors assignedto ‘CYP 2C9*2’6. If evaluating multiple loci, repeat steps 3 through 5 to create additional markersas needed.IMPORTANT! You must configure a marker with two detectors before you canapply it to a plate document.Note: Click for information on the features of the Marker Manager dialogbox or to delete markers from the Markers list.7. Apply the marker(s) to the allelic discrimination plate document as explained in“Copying and Applying Markers” on page 3-15.3-14 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Run.fm