Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 4Operating the InstrumentAbout the Centrifuge SystemAfter the fill reservoirs of a Low Density Array have been loaded with the cDNAsample or control, the centrifuge system is required to distribute the cDNA sample orcontrol to the reaction wells. The centrifuge system supports the simultaneousloading of up to 12 Low Density Arrays.RequiredCentrifuge andAccessoriesTo properly centrifuge the Low Density Arrays, you will need the followingcentrifuge and centrifuge accessories:• Sorvall ® Legend T CentrifugeGR2170Figure 4-7Sorvall Legend T Centrifuge• Sorvall ® /Heraeus “Tool-less” 750-mL Swing-Out Rotor Body• A set of Sorvall ® /Heraeus Custom Buckets (four), each with its own card holder(Contact your Applied Biosystems representative to order the custom buckets.)• Current firmwareIf you are upgrading an existing Sorvall Legend T or Legend RT Centrifuge,you may also need to upgrade the firmware so that it can recognize the LowDensity Array bucket type.CentrifugeSystemComponentsFigure 4-8 illustrates the centrifuge system components.Centrifuge rotorProvides the rotational drive fora set of four centrifuge bucketsCard holderSupports the Low DensityArray’s fill reservoirs duringcentrifugationGR2152GR2153GR2154BucketContains the Low DensityArrays and card holderduring centrifugationFigure 4-8Centrifuge System Components4-12 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Basic.fm