Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

detectorendogenouscontrolendogenousreferenceend-point(plate read)run/analysisexpression foldvaluehands-offautomationhigh throughput(HT)holdlocusmarkermethodA virtual representation of a gene- or allele-specific nucleic acid probe reagent usedfor absolute quantification, relative quantification, allelic discrimination, or otheranalyses performed on an SDS instrument. Examples of reagents represented asdetectors include TaqMan probes and the SYBR Green 1 dsDNA Binding Dye.Detector for a gene presented at a consistent level in all experimental samples. Byusing an endogenous control as an active reference, one can normalize quantificationof a messenger RNA (mRNA) target for differences in the amount of total RNA addedto each reaction.See endogenous control.Refers to processing of samples for allelic discrimination and plus/minus scoringchemistries of samples by the 7900HT instrument. The 7900HT instrument collects asingle reading after prior PCR temperature cycles are completed using a thermalcycler. After analysis by the SDS software, the resulting multi-component data is usedto assess the presence of target sequences in the unknown samples.The expression of a target gene relative to control gene in one sample, relative to theexpression level in a calibrator sample. See ∆∆C T .After plates have been loaded into the Zymark ® Twister Microplate Handler stacks,and the Automation Controller Software has been told to start running, no humanintervention should normally be required until all plates in the robot stacks have beenprocessed. With the SDS Enterprise Database option, this includes plate documentanalysis and analysis results persistence to the database.Describes the ability to analyze a large quantity of samples for genotyping or geneexpression analysis.A thermal cycling set-point of a method that maintains a specified temperature for anextended period of time.The physical location of a site on a chromosome, sometimes understood as the site ofa gene, usually understood as the site of a marker variant.For SDS software, a virtual representation of a variable segment of DNA (such as aSNP) whose inheritance can be followed, comprising a name and a set of two detectorsspecific for the allelic variants. Markers are used only for allelic discriminationassays, not for absolute quantification, or relative quantification. Allelicdiscrimination plate documents must contain at least one marker. The marker must beconfigured with two detectors before it can be applied to a plate document.The protocol that the Applied Biosystems 7900HT Fast Real-Time PCR Systemfollows during a real-time or end-point sequence detection run. Methods used by the7900HT instrument make use of the following information: thermal cycling times andtemperatures, ramp rates, auto-increment values, and data collection settings.Glossary-4 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, Glossary.fm