Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Analysis ChecklistAnalysis ChecklistWorkflowOverview 1. Open the plate document (see page 6-10).2. Configure the analysis settings for the run (see page 6-11).3. Analyze the run data (see page 6-12).4. For each detector that you configured to determine the baseline and thresholdvalues (see page 6-46):– Automatically – Verify that the baseline and threshold values for the detectorwere set correctly by the software.– Manually – Set the baseline and threshold values for the detector5. Visualize outliers and eliminate any outlying amplification from the run data(see page 6-49).6. View the results of the absolute quantification run (see page 6-14).7. Choose from the following post-analysis options (see page 6-52):– Reanalyze the run data.– Adjust the display settings for the results table, plate grid, and platedocument plots.– Print elements of the plate document.– Export the plate document results table or plots.– Save the analysis.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 6-9DRAFTSeptember 1, 2004 11:39 am, CH_Real-Time.fm