Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 7Maintaining the Instrument8. Create a pure dye plate for the custom dye(s) by pipetting each custom dye to atleast three columns of an optical plate at the concentrations determined instep 7.GR2107Custom Dye 1Custom Dye 2Custom Dye 3Custom Dye 4Custom Dye 5Custom Dye 6Custom Dye 7Custom Dye 8IMPORTANT! The optical configuration of the 7900HT instrument requires thateach pure dye occupy at least three columns of the Pure Dye plate to permitadequate data collection.9. Seal the plate using an optical adhesive cover or optical flat caps.10. Create a plate document template for the custom Pure Dye plate as explainedbelow.Constructing aCustom Pure DyePlate DocumentTemplate1. Start the SDS software.2. Add the new dye to the software using the Dye Manager:a. Select Tools > Dye Manager.b. In the Dye Manager dialog box, click .c. In the Add Dye dialog box, enter a name for the custom dye, andclick .The software adds the new dye to the Custom dye list.d. Repeat steps b and c to add any additional custom dyes to theDye Manager.e. Click .The SDS software makes the new dyes available to pure dye plate documents.3. Create a custom pure dye plate document for the run:a. Click (or select File > New).b. Configure the New Document dialog box with the following options:Assay – Select Pure Spectra.Container – Select the appropriate plate format.Template – Select Blank Template.c. Click . The software creates a new plate document.7-28 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm