Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 7Maintaining the InstrumentReplacing theSample BlockIMPORTANT! Before changing the sample block, perform all required upgrades tothe SDS software and instrument firmware. Failure to upgrade the software canrender the instrument inoperable or result in damage to instrument components.1. Load the sample block into the instrument compartment:a. Being careful not to damage the heat sinks on the bottom of the sampleblock, rest the sample block on the metal runners on either side of theinstrument bay.b. Carefully slide the sample block into the instrument until the front of theblock is flush with the rear of the locking bar.c. After it is seated, firmly press on the sample block to ensure a goodconnection.2. Replace the sample block locking bar.3. Tighten the thumbscrew (from step 9 on page 7-9) to secure the sample blocklocking bar to the instrument chassis (may be a 5/32-inch hex bolt).4. Using the 5/16-inch hex key, turn the sample block locking bolt clockwise untilit is flush with the locking bar.5. Again, press on the right and left sides of the front surface of the sample blockto ensure that it is seated securely.6. Replace the thermal cycler access cover:a. Fit the lip at the bottom of the access cover over the lower edge of the bay.b. Push the cover towards the instrument until it snaps into place.IMPORTANT! You must reinstall the thermal cycler access cover before poweringon the instrument. Failure to do so prevents the instrument from uploading thefirmware from the computer and causes the SDS software to display an error.GR2023bGR2025bGR20277-10 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm