Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Section 4.3 Automated OperationSection 4.3Automated OperationIn This Section Operating the Software with an SDS Enterprise Database . . . . . . . . . . . . . . . . . . 4-32Operating the Software without an SDS Enterprise Database . . . . . . . . . . . . . . . 4-34Operating the Software without an SDS Enterprise Database . . . . . . . . . . . . . . . 4-39After the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-44Pre-RunChecklistThe following tasks must be complete to run plates on the 7900HT instrument. Seethe associated page number for details on each procedure.Done Check See PageA background run has been performed in the last week 7-16A pure dye run has been performed in the last 6 months 7-20The instrument tray does not contain a plateIMPORTANT! The instrument tray must be empty to begin a run. Ifthe instrument tray contains a plate, you must eject and remove it.4-44The output stack does not contain plates. –OverviewThe Applied Biosystems 7900HT Fast Real-Time PCR System features the capacityfor high-throughput unattended operation through the use of the AutomationController Software. The Automation Controller Software coordinates the functionof the 7900HT instrument, the fixed-position bar code reader, and the ZymarkTwister Microplate Handler to run batches of prepared plates with minimal userintervention. The Automation Controller Software can run plates using platedocuments stored locally on the computer attached to the instrument, or across anetwork on an SDS Enterprise Database.Options for Automated OperationAs with the SDS Software, the Automation Controller Software requires the creationof a plate document for each plate it runs. The Automation Controller Software canrun batches of plates using plate documents saved to the hard drive of the computerattached to the instrument, or stored on an SDS Enterprise Database.Follow the appropriate procedure for your system:Operating the Software with an SDS Enterprise Database . . . . . . . . . . . . . . . . . . 4-32Operating the Software without an SDS Enterprise Database . . . . . . . . . . . . . . . 4-34Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 4-31DRAFTSeptember 1, 2004 11:39 am, CH_Basic.fm