Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

sample block locking bar 7-8sample block locking bolt 7-8sample block moduleabout 1-5cleaning sample block module wells 7-15contamination 8-7replacing 7-6sample namesadding to a plate document 3-28importing into a plate document A-2Sample readout (from the Real Time tab) 4-27sample volume setting 3-22saturation, signal 7-27savingplate documents 2-17, 4-24template files 3-24scrutinizing allele calls 5-16SDS 7900HT Document (*.sds) filesSee plate documentsSDS 7900HT Template Document (*.sdt) filesSee templatesSDS Enterprise Databaseabout 1-22command line tools 1-26RQ Manager Software 1-25saving plate documents 4-24saving results 5-19, 6-53saving templates 3-25SDS Database 1-26SDS User Account Manager 1-27SNP Manager Software 1-25study-centric design 1-27SDS softwareabout 1-14disconnecting 4-30installing 7-55launching 2-7learning to use the software 2-10re-connecting 4-30upgrading 7-55Sealerinstallation 4-14overview 4-14Sealer, see microfluidic card sealerSealingguidelines 4-19TaqMan Low Density Array 4-19trimming fill reservoirs after sealing 4-21selectingwells from the plate grid 2-24Service Name A-22setup table fileabout A-4example files A-4exporting A-17importing into a plate document A-2structure A-4signal saturation 7-27Slope readout (from the Standard Curve Plot) 6-13SNP Manager Software 1-25spectral calibration, See pure dye runsStage readout (from the Real Time tab) 4-27Standard Curve Method 6-4Standard Curve Plotabout 6-13exporting as a graphic file A-16exporting data as a text file A-16standardsquantifying for absolute quantification B-6selecting for absolute quantification B-6selecting for relative quantification B-6startingplate queue 4-43run from the SDS software 4-25State readout (from the Real Time tab) 4-27Status readout (from the Real Time tab) 4-27stepadding to a method 3-21Step readout (from the Real Time tab) 4-27stoppingplate queue 4-44run from the SDS software 4-28studies 1-28study-centric design 1-27SYBR Green 1 Dyeabout D-3System performance, verifyingRNase P card, preparing 7-32Ttable paneabout 2-29exporting A-16TaqMan fluorogenic probeabout D-2designing B-2 to B-3TaqMan Low Density Arraycentrifuging 4-16components 4-10handling 4-14how it works 4-10loading 4-14loading guidelines 4-14number of targets per well 4-10overview 4-10placing in bucket 4-16quality control 4-12removing from packaging 4-14sealing 4-19Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideIndex-7DRAFTSeptember 1, 2004 11:38 am, 4351684AIX.fm