Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Preparing TaqMan Low Density Arrays for Use2. Remove the sealed Low Density Array by grasping its sides and lifting it off thesealer’s insert plate.Note: In the middle of the sealer’s insert plate, there is a thumb slot to help youeasily access one side of the Low Density Array.Thumb slotGR21753. Inspect the Low Density Array for proper sealing. The indentations from thestylus assembly should match up with the Low Density Array’s main channels.If the indentations do not match up or if the foil is in any way damaged, do notuse the Low Density Array.4. Return the carriage to its starting position on the base of the sealer.IMPORTANT! Do not move the carriage back before removing the Low DensityArray.Trimming Off theFill Reservoirs1. Using scissors, trim the fill reservoirs from the Low Density Array. Use the edgeof the Low Density Array’s carrier as a guide.PHYSICAL INJURY HAZARD. Take care when trimmingoff the fill reservoirs. Use scissors rather than razor blades or other unprotectedcutting devices.GR2168The Low Density Array is now ready to be run on the 7900HT instrument.2. Choose from the following. If performing:– Absolute or Relative Quantification, or Dissociation Curve AnalysisRun the plate as explained in “Running the Plate” on page 4-25.– Allelic Discriminationa. Load the plate onto a designated thermal cycler and perform the PCR.b. Briefly centrifuge the plate to draw the reactions to the bottom of the wells andto eliminate any air bubbles that may have formed during thermal cycling.c. Run the plate as explained in “Running the Plate” on page 4-25.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 4-21DRAFTSeptember 1, 2004 11:39 am, CH_Basic.fm