Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 2Getting StartedTabsPlate documents can have up to six tabs (depending on their function) as describedbelow:Table 2-5Tabs of the SDS SoftwareTab Function Views/PlotsSetupInstrumentRaw DataPlotCalibrationDataResultsDissociationCurveDisplays well information, and allows youto configure the plate grid with setupinformation.Used to program the plate documentmethod, run the plate document, or send itto the Plate Queue.Displays the raw fluorescence collectedfrom the sequence detection run.The Raw Data tab is visible only in platedocuments containing run data.To view the tab, open a plate documentcontaining run data, click , then selectthe Raw Data Plot tab.Displays the Background and Pure Spectracalibration data used for the signalnormalization and multicomponentinganalysis of the run data.The Calibration Data tab is visible only inplate documents containing run data.To view the tab, open a plate documentcontaining run data, click , then selectthe Calibration Data tab.Displays analyzed run data.The Analysis tab is visible only in platedocuments containing analyzed run data.Displays analyzed dissociation curve datafrom a programmed ramp.The tab is visible only in plate documentscontaining analyzed data from a real-timerun with a programmed ramp.Well Inspector• Method Editor– Thermal Profile tab– Auto Increment tab– Ramp Rate tab– Data Collection tab• Real-Time tab• Plate-Read tabRaw Data PlotCalibration Data• Background Plot• Pure Dyes PlotAllelic Discrimination Data• Allelic Discrimination PlotAbsolute Quantification• Amplification Plot• Standard (Curve) PlotRelative Quantification• Amplification PlotDissociation Plot2-30 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:38 am, CH_GetStart.fm